Superoxide-mediated electrotaxis depends on the activation of Akt and Erk1/2.

<p>DCEF would induce the phosphorylation of Erk1/2 and Akt, and the activation of Erk1/2 or Akt were reduced by 20 µM PD98059 (inhibitor of Erk) or 20 µM LY294002 (inhibitor of Akt) respectively (A). DCEF-induced directional migration of U87 cells was also significantly decreased by PD98059 (B) or LY294002 (C). Bar = 25 µm.