Vpu inactivation affected distribution of Env in HeLa cells.

<p>HeLa cells were transfected with WT and mutant plasmids (L30E, ΔVpu, L30E-ΔVpu). At 24 h post-transfection, cells were washed, fixed, permeabilized and immunolabeled with anti-Env antibody, 2F5. Nuclei were stained using ProLong Anti-fade DAPI (Molecular Probes, Invitrogen). Cells were visualized using Olympus IX51 microscope at 60X oil immersion objective. Scale bar: 10 µm.