PKG II blocks the phosphorylation of PLCγ1.

<p>AGS cells were grown in 100-mm plates and infected with either Ad-LacZ or Ad-PKG II. Then, the cells were serum-starved o/n and treated differently: in Ad-LacZ group, no drug treatment; in Ad-LacZ+EGF group, the cells were incubated with EGF(100 ng/ml) for 5 min; in Ad-PKG II+ cGMP+EGF group, cells were incubated with 250 µM 8-pCPT-cGMP for 1 h and followed by incubating with EGF (100 ng/ml) for 5 min. Immunoprecipitation with antibody against PLCγ1 was performed to precipitate PLCγ1 and the phosphorylation of precipitated PLCγ1 was analyze by Western blotting with antibody against phospho- PLCγ1(Tyr783). The results shown are representative of three independent experiments.