The ability to up-regulate <i>gem</i> and <i>zic2</i> is lost in the AB4 mutant.

<p>(A) The FoxD4L1-AB mutant expressing clones, marked by nuclear βGal (pink dots), are located in the neural ectoderm. For AB1 and AB2, the βGal labeled cells are more intensely stained (darker blue) than their neighboring cells (e) expressing endogenous level of <i>gem</i> or <i>zic2</i>. For AB4, the βGal labeled cells are stained at the same intensity as the neighboring cells (e). Insets are higher magnifications of the clone, the position of which is indicated on the whole embryo by a bracket. For <i>gem</i>, images are dorsal views with vegetal pole to the top; for <i>zic2</i>, images are vegetal views with dorsal to the top. (B) The percentage of embryos in which the FoxD4L1-AB mutants caused up-regulation of <i>gem</i> or <i>zic2</i> in the dorsal neural ectoderm. The data for the ΔAB mutant (14aa deletion in Fig. 8A) is shown for comparison. Numbers above each bar indicates sample size; * indicates significant difference from wild type (WT) at the p<0.001 level. Data for WT and ΔAB are from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061845#pone.0061845-Neilson1" target="_blank">[39]</a>.