Gfi1 is a downstream target of SH-2251.

<p><b>(A)</b>, A decreased <i>Gfi1</i> mRNA expression in the SH-2251 treated Th2 cells. The expression of <i>Gfi1</i> was determined with quantitative RT-PCR. The relative intensity (/<i>Hprt</i>) is shown with the standard deviation. <b>(B)</b>, A decreased level of Gfi1 proteins was detected with immunoblotting. The nuclear (Gfi1) and cytoplasmic (α-Tubulin) lysates with a three fold serial dilution were used. <b>(C)</b>, The global patterns of the histone H3K4me3 and H3K27ac levels at the <i>Gfi1</i> gene locus were determined using ChIP-sequencing. The locations of PCR primer pairs (triangle) used in a manual ChIP assay are also listed. <b>(D)</b>, Manual ChIP assays were performed with anti-histone H3K4me3, H3K9ac or H3K27ac pAb. The relative intensity (/Input) is shown with the standard deviation. Four independent experiments (A, B and D) were performed with similar results.