Inhibition of acidosis/GPR4-induced cAMP production by the GPR4 antagonist in HUVEC.

<p>(A–B) HUVEC/Vector and HUVEC/GPR4 cells were treated with varying pH in the presence or absence of the GPR4 antagonist EIDIP. After the pH treatment, intracellular cAMP was measured as described in the Materials and Methods. The vehicle control had 0.04% DMSO which is the same DMSO concentration as that in 20 µM GPR4 antagonist. The results are the average of 10 samples for HUVEC/Vector cells and 7 samples for HUVEC/GPR4 cells. Error bars indicate the mean ± SEM. **, <i>P</i><0.01; ***, <i>P</i><0.001; <i>ns</i>, not significant.