Expression of iNOS can be detected in beta-TC6 cells.

<p>(A) Non-GFP positive beta-TC6 cells were either cultured alone (left panels) or together with GFP-positive NCSC cells (right panels, green cells) on laminin-coated cover slips. Cells were treated with 20 ng/mL of IL-1β and 20 ng/mL of IFN-γ for 48 hours. Cells were then fixated, permeabilized and stained for iNOS (red). Nuclei were visualized by Hoechst. Fluorescence was then photographed in a fluorescence microscope using a 40× lens. The white scale bar depicts a distance of 100 µm. Results are representative for three independent experiments. (B) Immunoblot analysis of NCSCs (left panel) and beta-TC6 cells (right panel). Both cell types were cultured alone. Cells were stimulated for 12 hours with IL-1β and IFN-γ and then analysed for iNOS expression by immunoblot analysis. As loading control tubulin was used. The results are representative for three observations.