Dynamics of differentially expressed miRNA after irradiation in CL1-0 and CL1-5.

<p>(A) Hierarchical clustering of differentially expressed miRNA from CL1-0 and CL1-5 treated with 10 Gy. Cells were harvested respectively at 0, 1, 4, 24 h after irradiation. (B) Principal component analysis (PCA) of the radiation-responsive genes. The axes are the first two principal components (PC), which can explain most of the miRNA expression profiling. Three independent experiments were done at each time point. Different colors represent different cells; different shapes represent different time points. (C) Relative miR-449a expression level measured by microarray. Data were the mean ± SD for three independent experiments. *: <i>P</i><0.05. (D) Relative miR-449a expression level measured by quantitative RT-PCR. Data were the mean ± SD for four independent experiments.