Characterization of the translocants between homologs and proposed model explaining the role of Rdh54.

<p>A) Characterization through colony-PCR of cl44 and cl94. The presence of spacer2 (lane 1 = primers sp2/sp2Fw), spacer3 (lane 2 = primers sp3/Dur3’int) and spacer1 (lane3 = primers Sp1/Dur3R) are shown from left to right in the wild type strain YF123, in the translocant 44 (<i>rdh54Δ</i>/<i>rdh54Δ</i> background) and in the translocant 94 (wt YF123) respectively. In the panel on the right the amplification size of the region surrounding spacer3 in clone 44 and 94 is compared (DurEXT/Dur3’int). The partial sequence of these fragments is shown in details on panel C. B) Two hypothetical models to explain the loss or the retaining of spacer sp3 in the wild type cl 94 (I, II) and in the <i>rdh54Δ</i>/<i>rdh54Δ</i>cl 44 respectively (III, IV). C) DNA consensus from the alignment of a partial sequence of cl44 and cl94 around spacer3 confirming that only in the first one the spacer is present (here indicated as green, small letters). The capital letters indicate the nucleotides present in both clones.