Resolved sequence structures for the 7 <i>rrn</i> operons in MRSN 2154.

<p>PCR and Sanger sequencing were used to determine the variable regions in <i>rrn</i> operons. RC, reversed and complementary. (<b>A</b>) Illustration of contig assembly for the 7 <i>rrn</i> operons and adjacent sequences. Variable regions between rRNA sequences (contigs 41, 45 and 51) were shown in <i>orange</i> boxes and indicated as orientation-undecided by the <i>empty arrow</i>. The sizes for the ribosomal and intergene regions are shown. (<b>B</b>) Schematic representation of regions to be amplified and the Sanger sequencing directions. Seven <i>rrn</i> regions were amplified by PCR using 7 pairs of primers, FP1-FP7 and RP1-RP7 which are corresponding to specific sequences flanking the <i>rrn</i> regions. Sanger sequencing outward from the conserved rRNA sequence (contig 51) in both directions with primers P51R and P51F was used to determine the intergene spacer regions. (<b>C</b>) The results for <i>rrn</i> operon sequence structures resolved by PCR amplification and Sanger sequencing.