Methylation patterns of DNMTs and tumor suppressor genes in normal and cancerous ovaries from laying hens.

<p>[A and B] Localization of 5-methylcytosine protein in normal and cancerous ovaries of hens. Sections were not counterstained. Arrows in panel B indicate nuclei in the glandular epithelium of ovaries. [C] Methylation status of promoter regions of tumor suppressor genes using methylation-specific PCR analyses. Legend: GE, glandular epithelium; M, methyl primer; U, unmethyl primer. See <i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061658#s4" target="_blank">Materials and Methods</a></i> for a complete description of the methods.