The N1 Region of CSB Is Critical for NAP1L1 Interaction.

<p>(A) Schematic illustration of CSB and CSBΔN1 used in assays. (B) Western blot analysis of GST pull-down assays to examine CSB and NAP1L1 interactions. Purified CSB or CSBΔN1 were mixed with immobilized GST or GST-NAP1L1. Western blots showing levels of input CSB and CSBΔN1 as well as their binding to GST-NAP1L1. (C) Immunoprecipitation using an anti-NAP1L1 antibody to examine the CSB- and CSBΔN1-NAP1L1 interactions in cells, with or without UV treatment (100 J/m²).