Expression profiling of protein phosphatases and cell cycle proteins in different PCa cells.

<p>LNCaP C-33, C-81 and PC-3 PCa cells were plated at a density of 8×10³, 6×10³ and 4.8×10³ cells/cm², respectively, in duplicates for 3 days in regular medium. (Left FBS panel, lanes #1–3) Cells were replaced with fresh regular medium for 24 hr and then harvested. (Right SR panel, lanes #4–6) All three PCa cells were then steroid-starved for 48 hr in a steroid-reduced (SR) medium and then harvested. Total cell lysate proteins were analyzed for cPAcP, SHP1, SHP2, Cdc25A, Cdc25B, Cdc25C, Cyclin B1 and Cyclin D1. Cdc25C spliced forms were observed upon long exposure of films (lower panel of Cdc25C). β-actin was analyzed and used as a loading control.