Generation of <i>Ttda</i> knock-out mice.

<p>(A) Schematic presentation of the mouse <i>Ttda</i> genomic locus, <i>Ttda</i> targeting construct and <i>Ttda</i> locus after CagCre recombination. Roman numbered boxes represent exons: white boxes are non-coding exon (parts) and black boxes are coding exon (parts). In the targeting construct LoxP sites are indicated with gray arrows and the dashed box represents the Neomycin selectable marker driven by a PGK promoter. The positions of NheI restriction sites, size of the NheI restriction fragments and the position of probe A used for DNA blot screening of digested ES cell DNA are indicated. The short arrows indicate the position and direction of primers used for genotyping. (B) DNA blot analysis of NheI-digested ES cell DNA using the probe A. (C) Genotype analysis with diagnostic PCR using primers 1F, 1R, 2R and NeoR in the different combinations (A, B and C).