Survival rate, clinical score and vascular leakage in DENV-infected mice treated once or three times with 7.5% NaCl.

<p>Groups of 8–9 week-old AG129 mice were sc. infected with D2Y98P-PP1 strain. At day 6 p.i., they were iv. administered with a volume (4 ml/kg body weight) of sterile 7.5% NaCl (HTS) or 0.9% NaCl (PBS) once (D6), or once for three consecutive days (D6–D8) as indicated. (A) Survival rate (n = 10). (B) Clinical scores (n = 10): 0: Healthy; 1: Ruffled fur; 2: Hunched back; 3: Severe Diarrhea; 4: Moribund stage; 5: Severe weight loss. (C) Systemic vascular leakage in infected mice. Three hours post-HTS or PBS treatment, DENV-infected mice (n = 5) were assessed for vascular leakage and the quantity of Evan’s blue dye was determined in various organs from the individual mice as indicated. Results are expressed as fold change compared to uninfected untreated control mice. (D) Systemic vascular leakage in uninfected mice. Uninfected mice (n = 5) were subjected to HTS treatment (once or once daily for three days) followed by Evan’s blue assay 3 hrs after the last administration.