In vivo fluorescence images of mouse tumors model with cy5.5 solutions.

<p>Mice with average tumor sizes of 0.8×0.6 cm (marked with red circles with arrows in left panels) were injected intravenously with: a) cy5.5 free acid+nanobubbles; b) cy5.5-choitosan solution as a control of shell materials without nanobubble structure; and c) cy5.5-nanobubble suspension. For panels b and c, cy5.5 fluorescence emission is shown under the same scale of radiation efficiency for comparison of intensity at the tumor sites. Fluorescence images are shown as overlaid fluorescence emission profiles on photographic images.