Co-culture of JFH-1-infected Huh7.5.1 cells and pDC-GEN2.2 cells leads to the upregulation of IFN genes and viral control.

<p>A) Huh7.5.1 cells were infected for 24 hours with JFH-1 and then resting pDC-GEN2.2 cells were added for 24 hours. mRNA from the CD45+ cells was isolated and examined for IFN gene expression. Gene fold increase is shown for each gene after normalization to the reference gene GAPDH and the uninfected condition. B) Huh7.5.1 cells were infected for 24 hours with JFH-1 and then pDC-GEN2.2 cells that had been mock transfected, transfected with X-region RNA or pU/UC RNA for 8 hours were added for 4 days (5 days total infection). RNA was isolated and examined for JFH-1 copy number. Normalized HCV copy number is shown where the infection control condition HCV copy number is set to 1 and other conditions are expressed as normalized HCV copy number compared to infection control. Normalized HCV Copy Number = (Absolute copy number for condition/absolute copy number for infection control) p values represent the Mann-Whitney result of the comparison. * p<0.05 ** p<0.01 *** p<0.001 # p≤0.0001. Bars represent the mean and error bars are +/− SEM.