Titers of VV-specific IgG1 and IgM were increased in VV-infected AAD mice at 10 dpi.

<p>Serum levels of (A) OVA-specific IgE, (B) VV-specific IgG2a, (C) VV-specific IgG1 and (D) VV-specific IgM were determined by ELISA. Serum antibody levels were determined by antibody capture on OVA or virus coated plates with detection of bound antibodies via enzyme linked secondary reagents as outlined in the methods. (A) OVA-specific IgE levels from mouse serum are reported as absorbance values. (B–D) VV-specific antibody titers were determined as endpoint titers 0.1 OD unit more than background (PBS/1% BSA). Statistical significance was determined by a One-way ANOVA with Bonferroni’s multiple comparisons test: NS – not significant; ^###<i>P</i><0.001, ^####<i>P</i><0.0001 AAD+VV vs. VV. Results are expressed as the mean ± SEM for 5–6 mice in each group and are representative of 3 independent experiments.