<p>(a) Effect of pH on enzymatic activity, measured in 50 mM glycine-HCl buffer (pH 2.5–3.5, closed circles), HAC-NaAC buffer (pH 3.5–6.0, open circles) and Na phosphate buffer (pH 6.0–8.0, triangles) at 37°C for 30 min, the sucrose was 10 g l<sup>−1</sup> in a volume of 0.5 ml. Enzyme activity is shown as the specific activity: one unit represents 1 µmol of glucose released from the reaction per min per mg protein. (b) Effect of temperature on the activity of uninv2 (dashed line) and M-inv2 (solid line). The temperature dependence of the enzymes’ activity was measured between 20°C and 60°C under the optimum pH condition (pH 4.5 or 6.0 for uninv2 and M-inv2, respectively). The error bars represent the standard deviation of triplicate measur...
<p>(A) Determination of optimum pH. The hydrolytic activity was measured at different pHs at 40°C fo...
<p>Enzyme was incubated in 200 mM phosphate-citric acid-glycine buffer containing 0.5% (w/v) of CMC ...
<p>(A) Optimal temperature, (B) thermostability assay, (C) optimal pH, (D) pH stability assay. The e...
<p>Effect of (a) pH on enzyme activity, (b) pH on enzyme stability; pH 3.0 (♦), pH 4.0 (□), pH 5.0 (...
<p>(a) Effects of temperature, from 20°C to 45°C, on enzyme activity in the presence of Mg<sup>2+</s...
<p>The enzyme activities were measured using <i>p-butyrate caprylate (C<sub>6</sub>)</i> as substrat...
<p>(A) The effects of temperature. (B) The effects of buffer and pH value. All the reaction mix (100...
<p>The pH stability of uninv (a), uninv2 (b) and M-inv2 (c) were tested at 37°C. The protein was inc...
<p>(<b>A</b>) Effect of pH on the enzymatic activity of TpManGH5 at 75°C. (<b>B</b>) Effect of tempe...
<p>Enzyme reactions were performed under standard conditions in the presence of 15 mM M6P. Activitie...
<p><b>A:</b> Temperature dependent enzyme activity profile assayed at various temperatures (20–60°C)...
<p>(a) Relative activity at different temperatures; and (b) relative activity at different pH. Relat...
<p>A range of temperature (A) and pH (B) conditions were examined using substrate Gly-pro-AMC. To de...
<p>A, The temperature activity profile; B, pH dependence reactions catalyzed by PH1704; Substrate sp...
Relative activity of (A) CalB (B) Commercial lipozyme CalB from Siam Victory Chemicals (C) SeqA and ...
<p>(A) Determination of optimum pH. The hydrolytic activity was measured at different pHs at 40°C fo...
<p>Enzyme was incubated in 200 mM phosphate-citric acid-glycine buffer containing 0.5% (w/v) of CMC ...
<p>(A) Optimal temperature, (B) thermostability assay, (C) optimal pH, (D) pH stability assay. The e...
<p>Effect of (a) pH on enzyme activity, (b) pH on enzyme stability; pH 3.0 (♦), pH 4.0 (□), pH 5.0 (...
<p>(a) Effects of temperature, from 20°C to 45°C, on enzyme activity in the presence of Mg<sup>2+</s...
<p>The enzyme activities were measured using <i>p-butyrate caprylate (C<sub>6</sub>)</i> as substrat...
<p>(A) The effects of temperature. (B) The effects of buffer and pH value. All the reaction mix (100...
<p>The pH stability of uninv (a), uninv2 (b) and M-inv2 (c) were tested at 37°C. The protein was inc...
<p>(<b>A</b>) Effect of pH on the enzymatic activity of TpManGH5 at 75°C. (<b>B</b>) Effect of tempe...
<p>Enzyme reactions were performed under standard conditions in the presence of 15 mM M6P. Activitie...
<p><b>A:</b> Temperature dependent enzyme activity profile assayed at various temperatures (20–60°C)...
<p>(a) Relative activity at different temperatures; and (b) relative activity at different pH. Relat...
<p>A range of temperature (A) and pH (B) conditions were examined using substrate Gly-pro-AMC. To de...
<p>A, The temperature activity profile; B, pH dependence reactions catalyzed by PH1704; Substrate sp...
Relative activity of (A) CalB (B) Commercial lipozyme CalB from Siam Victory Chemicals (C) SeqA and ...
<p>(A) Determination of optimum pH. The hydrolytic activity was measured at different pHs at 40°C fo...
<p>Enzyme was incubated in 200 mM phosphate-citric acid-glycine buffer containing 0.5% (w/v) of CMC ...
<p>(A) Optimal temperature, (B) thermostability assay, (C) optimal pH, (D) pH stability assay. The e...