Histone modifications associated with <i>BMP2</i> expression.

<p>A. qRT-PCR of <i>BMP2</i> expression in BMSCs from SD rats treated with varying concentrations of TSA. B. qRT-PCR of <i>BMP2</i> expression in BMSCs from SD rats treated with varying concentrations of TSA followed by 1,25(OH)₂D₃. C. qRT-PCR of <i>BMP2</i> expression in BMSCs from GHS rats treated with varying concentrations of TSA. D. qRT-PCR of <i>BMP2</i> expression in BMSCs from GHS rats treated with varying concentrations of TSA followed by 1,25(OH)₂D₃. E. qRT-PCR of <i>BMP2</i> expression in UMR-106 cells treated with varying concentrations of TSA. F. qRT-PCR of <i>BMP2</i> expression in UMR-106 cells treated with varying concentrations of TSA followed by 1,25(OH)₂D₃. G. Antibody against acetyl-histone H3 was used to do ChIP assay on chromatin extracted from UMR-106 cells which were incubated with 1,25(OH)₂D₃ (together with or without TSA) or vehicle. The normal IgG was used as a negative control. Primer pairs designed for <i>BMP2</i> promoter region C were used in the PCR. (*, P<0.05; **, P<0.01).