Insulin metabolism and insulin resistance markers in mice.

<p>Evaluation of adiponectin in Ctl (white bars) and C5L2^−/− (black bars) adipocyte primary cell culture media for 24 h isolated from inguinal (A) and perigonadal (B) adipose tissue after 6, 12 and 24 weeks on chow diet and DIO. Data are presented as means±SEM (3–10 adipose tissue media per group) over time and expressed as pg/mL or ng/mL per gram (g) of tissue used. Retroperirenal adipose tissue mRNA gene expression for genes related to insulin resistance (C–D) from Ctl (circle and dotted line) and C5L2^−/− (square and solid line) mice on chow diet (white circle or square) and DIO (black circle or square). Data are expressed as relative expression (re) mean±SEM (n = 4–5 mice per group) compared to 6 weeks chow diet Ctl set as 1. Plasma levels of adiponectin (E), glucose (F) and insulin in Ctl (circle and dotted line) and C5L2^−/− (square and solid line) mice on chow diet (white circle or square) and DIO (black circle or square). Data are presented as mean±SEM (n = 8–10 mice per group) over time with two-way (2-way) ANOVA for genotype × time between Ctl and C5L2^−/− for each diet or adipose tissue with a Bonferroni post-test where * <i>p</i><0.05, ** <i>p</i><0.01 and *** <i>p</i><0.001 for C5L2^−/− vs. Ctl. Abbreviations: SOCS3, suppressor of cytokine signaling 3; NOS2, nitric oxide synthase 2.