Generation and detailed phenotypic analysis of seHAdV-T-cells.

<p>PBMCs (5×10⁶/12 well) were expanded for 12 days using the HAdV peptide pool and IL-15, as described in material and methods. A) The total number of PBMCs after expansion, and B) the absolute number of HAdV-multimer-specific T-cells at days 0 and 12, are shown from 24 donors, including mean +SEM and ranges. C) PBMCs (2.5×10⁶/24 well) were CFSE-labeled without stimulation (unst.), or stimulated with the HAdV-peptide pool in the presence of IL-15, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0059592#s2" target="_blank">Materials and Methods</a>. A summarizing diagram + SEM of 3 donors shows cell number (x1000) (bars) and percentage (above bars) of viable proliferating cells after expansion including a representative histogram.