IFNβ reduces mutant p53 mRNA levels by inhibiting its RNA stabiliser WIG1 (ZMAT3).

<p>Cells were treated with IFNβ for the designated time points, fixed and sorted by an “Image stream” FACS. (A) Representative images from each condition and (B) a graph representing the mean pixel intensity of mutant p53 positive cells for the entire population. (C) Cells were treated with the designated IFNs for 24 h and mutant p53 and GAPDH levels were measured by western blot. Cells were treated with IFNβ for 24 h and mutant p53 (D) and WIG1 (E) RNA levels were determined by QRT-PCR. * P<0.05. (F) HepG2 cells were introduced with mut175 plasmid and treated with IFNβ. RNA levels were determined by QRT-PCR. * P<0.05. (G) H1299 cell harbouring a TS form of mutant p53 (Wild type form at 32°C and mutant form at 37°C) were treated with IFNβ for 30 h. p53 and GAPDH levels were measured by western blot, shown in the lower panel a is normalized quantification of the bands.