Influences of PCN co-treatment on the hepatocyte proliferation induced by TCPOBOP treatment in PXR-deficient mice.

<p>Male <i>Pxr^−/−</i> mice were treated intraperitoneally with vehicle (corn oil; Control), TCPOBOP (TC; 3 mg/kg), PCN (100 mg/kg) or both for 48 h. (A) The liver to body weight ratios were calculated. (B) Livers were fixed and stained with anti-Ki-67 antibody. Arrowheads indicate Ki-67-positive nucleus. (C) The percentage of Ki-67-positive nuclei was calculated as described in Materials and Methods. (D) Total RNAs extracted from the liver were subjected to quantitative RT-PCR for <i>Cyp2b10</i> and <i>Ccnb1</i>. Values are the mean ± SD (n = 4). Columns not sharing a common letter (a and b) differ significantly with each other (<i>P</i><0.05; Tukey-Kramer test).