Depletion of ILCs with anti Thy-1 mAb exacerbates CCl₄-induced hepatitis in RAG-2^−/− mice.

<p>(A) Serum ALT levels of olive oil or CCl₄-injected RAG-2^−/− mice, or anti-Thy1 mAb and CCl₄ (Thy1 CCl₄) or anti-NK1.1 mAb and CCl₄ (NK1.1 CCl₄)-treated RAG-2^−/− mice 12 h after CCl₄ or olive oil injection. Data show the mean ± SEM (n = 9/group). (B) Representative photomicrographs of H&E-stained liver from each group and pathological score. (C) CD11b/CD11c staining of hepatic MNCs from each group 12 h after CCl₄ or olive oil treatment. Ratio and absolute number of CD11b⁺ macrophage in the hepatic MNCs. Data show the mean ± SEM (n = 5/group). (D) TCRβ/NK1.1 staining of hepatic MNCs. Ratio and absolute number of NK cells in the hepatic MNCs. Data show the mean ± SEM. (E) SCA-1/Thy1 staining of Lin⁻ hepatic MNC. Ratio of ILCs in the Lin⁻ fraction and absolute number of ILCs in each group. Data show the mean ± SEM. Data are representative of three independent experiments.