In vivo tumorigenicity and differentiation capacity of CD271⁺ cells.

<p>(A) Representative tumors in mice into which the indicated number of cells were transplanted. Red arrowheads indicate CD271⁺ cell injection sites (right side), and blue arrowheads indicate CD271⁻ cell injection sites (left side). Circles and dashed circles indicate transplantation locations resulting in success and failure of tumor formation, respectively. (B) Flow cytometry analysis of the sorted cells (96.8∼99.5% CD271⁺ or CD271⁻ cells). (C) Thirty CD271⁺ cells (right side) and CD271⁻ cells (left side) were transplanted into a mouse, and the generated tumors were resected. Tumor growth was plotted using the average value. (D) Flow cytometry analysis of the CD271 expression of tumor cells resulting from the injection of CD271⁺ cells. Tumor sections were stained with hematoxylin and eosin (H&E). Scale bar: 100 µm.