Analysis of two transcriptomes to isolate genes whose expression is upregulated by the interaction with macrophages.

<p><i>A</i>, The Venn diagram shows genes that are upregulated in the db/db white adipose tissue and down-regulated by vitamin B6 supplementation. Of a total 1810 genes up-regulated in db/db adipose tissue, the expression of 262 genes was decreased by dietary vitamin B6 (<i>p</i><0.05). <i>B</i>, Semiquantitative RT-PCR was performed to determine mRNA levels of <i>MMP3</i> and <i>PTX3</i>. The level of β-actin (<i>β-actin</i>) transcript was used as a control. <i>C</i>, <i>D</i>, Total RNA from individual mice (n = 4) in two groups was subjected to quantitative PCR to examine mRNA expression. All values are normalized to β-actin levels. The data (mean ± S.E.) are representative of two independent experiments. *<i>P</i><0.05 compared with those of db/+ mice. <i>E</i>,<i>F</i>, Total RNA from individual mice (n = 12) in two groups was subjected to quantitative PCR to examine mRNA expression level. All values are normalized to β-actin levels. The data (mean ± S.E.) are representative of two independent experiments. *<i>P</i><0.05 compared with those of 1 mg/kg PN (<i>1</i>).