Modification of PPA1 in rat cortical neurons.

<p>(A) Representative Western blot analysis (A) and quantitative analysis of PPA1 protein (B). PPA1 knock-down in rat cortical neurons using siRNA targeted to rat PPA1 (si-PPA1). As a control, si-RNA targeted to luciferase was used (si-luc) (n = 5). (C) A representative rat cortical neuron treated with si-RNA targeted to rat PPA1 and targeted to luciferase as a control. (D–E) Quantitative analysis of neurite growth in rat cortical neurons. Neurite growth is determined by morphological analysis as described in the Experimental procedure (n = 100). (F) GFP overexpression in rat cortical neurons using adenovirus containing GFP. (G and H) Representative Western blot analysis (G) and quantitative analysis of PPA1 protein (H). PPA1 overexpression in rat cortical neurons using adenovirus containing mouse PPA1 (PPA1). As a control, adenovirus containing GFP was used (GFP) (n = 5). (I) Representative rat cortical neurons treated with PPA1 adenovirus and GFP virus as a control. (J and K) Quantitative analysis of neurite growth from rat cortical neurons (n = 100). (L and M) A representative rat cortical neuron treated with 10 µM SP600126, an inhibitor of JNK (SP600125), and vehicle as a control (vehicle) (n = 5).*p<0.05 vs. si-luc, ^#p<0.05 vs. GFP. ^ap<0.05 vs. vehicle.