Gel Mobility Shift Assays illustrate that snail NSCaTE of LCav1 and mammalian NSCaTE of Cav1.2 can displace calcium-calmodulin (Ca²⁺-CaM) prebound to either snail IQ or mammalian IQ motifs.

<p>Gel shift mobility assays of CaM and individual peptides (A) or CaM pre-bound to an IQ peptide competing with increasing amounts of NSCaTE (B). Each lane contains 300pmol wild-type Ca²⁺-CaM; first lane is a CaM-only control for reference (Positions #2,#3,#5). (A) Each subsequent lane has increasing ratios of C-terminal (IQ) and/or N-terminal NSCaTE peptides at the ratios indicated. The changing conformation of CaM by IQ peptide causes a mobility shift of the CaM band (from Positions #2 → #1, top panels). Neither snail NSCaTE_LCav1 or mammalian NSCaTE_Cav1.2 changed CaM mobility alone, at any ratio (Position #3, bottom panels). (B) First lane is again Ca²⁺-CaM-only control (Position #5). Second lane is Ca²⁺-CaM with 1.5×IQ peptide alone control (for the maximum shift reference, Position #4). In subsequent lanes, increasing the amount of added NSCaTE peptide eventually reversed the slow mobility of CaM-IQ peptide back to the faster mobility of CaM without IQ peptide (Position #4 → #5), but not at a 100% effectiveness.