MBL alters the ability of ALD-DNA–stimulated macrophages to promote T cell differentiation.

<p>Peritoneal macrophages were incubated with PBS, ALD-DNA (50 µg/ml) or ALD-DNA (50 µg/ml) plus MBL (10 µg/ml) for 48 h. (A) The expression of MHC II, CD80 and CD86 was examined by flow cytometry and the levels of them were represented as mean fluorescent intensity (MFI). (B and C) These peritoneal macrophages and allogeneic T cells isolated from female BALB/c mice were cultured at a 1∶5 ratio in the presence of ALD-DNA (50 µg/ml) for 6 days. For intracellular analysis of cytokine production, T cells were pre-stimulated with 10 ng/ml phorbol myristate acetate and 1 µg/ml ionomycin in the presence of 10 µg/ml brefeldin A for 5 to 6 hours. Cells were then fixed, permeabilized, and stained for IFN-γ and IL-17 and analyzed by flow cytometry. Average of percentage of IFN-γ⁺ CD4⁺ or IL-17⁺CD4⁺ T cells was presented in the bar charts. Data are means ± SD of three independent experiments. **<i>p</i><0.01.