Tamoxifen interferes with TGF-β1-induced E-cadherin mRNA down-regulation and blocks the induction of Snail mRNA.

<p>Omentum-derived MCs were treated or not with 1 ng/mL TGF-β1 for 12 or 24 hours, in the presence of different doses of Tamoxifen (0, 3 and 6 µM). <b>(A)</b> Quantitative RT-PCR demonstrates that Tamoxifen treatments prevent TGF-β1-induced E-cadherin mRNA down-regulation. <b>(B)</b> Quantitative RT-PCR shows that Tamoxifen blocks TGF-β1-mediated induction of Snail mRNA, the main transcriptional repressor of E-cadherin. The expression of E-cadherin mRNA was analyzed at 24 hours, whereas the expression of Snail mRNA was analyzed at 12 hours of treatments. Bars depict means ± SE of three independent experiments.