Effects of Tamoxifen on the number of fibroblasts derived from MCs.

<p><b>(A)</b> Immunofluorescence microscopy analysis of parietal peritoneal sections stained for cytokeratin (green) and FSP-1 (red) with DAPI counterstaining show accumulation of trans-differentiated mesothelial cells in the submesothelial space (cytokeratin positive cells) in the PDF group, some of which co-express FSP-1 (yellow cells in the Merge panel). The administration of Tamoxifen reduces the number of cytokeratin/FSP-1 double positive cells per field. Representative slides are presented. Magnification ×200. <b>(B)</b> Reductions of the number of cytokeratin/FSP-1 positive fibroblasts by Tamoxifen are significant. Box Plots represent 25% and 75% percentiles, median, minimum and maximum values. Numbers above boxes depict means ± SE. Symbols show statistical differences between groups.