Stx16-depleted MDCK monolayer show decreased transepithelial resistance (TER).

<p>(<b>A</b>) Immunofluorescence staining for Stx16 expression in src-shRNA-MDCK and sh<i>Stx16</i>-MDCK cells. (<b>B</b>) Western blotting of Stx16 expression in src-shRNA-MDCK and sh<i>Stx16</i>-MDCK cells. (<b>C</b>) Quantitation of band densities from <b><i>B</i></b>. Values represent relative protein levels after normalization to an arbitrary value of 100% for src-shRNA expressing cells. The percentage represents the mean (±SD) for n = 3. (<b>D</b>) Transepithelial resistance (TER) measured in monolayers generated from src-shRNA-MDCK and shStx16-MDCK cells at indicated time intervals. (<b>E</b>) Recovery of TER in MDCK monolayers following a Ca²⁺-switch. Stx16 depletion caused a delayed recovery of TER. Graph represents the averages from 3 independent experiments (plus 3 replicates per experiment) and error bars show SEM.