The effect of leflunomide on ocular IL17 mRNA and IFN-γmRNA expression in EAU rats.

<p><b> </b> The mRNA levels were normalized to those of β-actin(<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062071#pone-0062071-g004" target="_blank">Figure 4A</a>). RT-PCR analysis reveals that the mRNA expression of IL17 and IFN-γwere significantly increased in IRBP-treated rats. In contrast, leflunomide treatment reduced the expression of IL17 and IFN-γin EAU rats(<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062071#pone-0062071-g004" target="_blank">Figure 4B,4C</a>). Data are expressed as the mean±SD (<i>n</i> = 8). *<i>P</i><0.05; **<i>P</i><0.01; ***<i>P</i><0.001. 1, normal; 2, vehicle; 3, low-dose leflunomide (3 mg/kg); 4, medium-dose leflunomide (6 mg/kg); 5, high-dose leflunomide (12 mg/kg); 6, CsA (10 mg/kg).