Immunocytochemical staining for ubiquitin in control (P29) and transected (P28+1 d) spinal cord of <i>Monodelphis domestica.</i>

<p>A: P29 (control). Note distribution of immunostaining for ubiquitin similar to P14 controls (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062120#pone-0062120-g005" target="_blank">Figure 5A</a>) but immunostaining of large ventral horn motor neurons and neurons (cells of Waldeyer) surrounding dorsal horn is more prominent. B: P28+1 d post injury. Note strong immunostaining of ventral horn motor neurons as in P29 control (A). Also many more neurons in dorsal horn grey matter show prominent immunostaining and intense nuclear staining of oligodendroglia in the presumptive white matter and strong staining of glial processes including those of radial glia. These features in the boxed area are shown at higher power magnification in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062120#pone-0062120-g008" target="_blank">Figure 8</a> B. Endothelial cells and neuroependymal cells lining the central spinal cord are negative. A and B, same magnification. Bar in B: 100 µm.