Overexpression of miR-200b and miR-200c inhibits expression of mesenchymal markers and increases E-cadherin in LY2 cells.

<p>(A–C) LY2 cells were not transfected (Non-TF), Mock-transfected (RNAiMAX), or transfected with negative control, pre-miR-200b, or pre-miR-200c for 24 h before preparing RNA or WCE for subsequent analysis. A, <i>ZEB1</i>, <i>ZEB2</i>, E-cadherin (<i>CDH1</i>), and vimentin (<i>VIM</i>) expression was quantified by qPCR. Values are the mean ± SEM. *p<0.05 versus negative control for each gene. B–C, Protein expression of Zeb1 as well as EMT markers N-cadherin, vimentin, and Slug, and epithelial marker E-cadherin was analyzed by western blotting normalized to the expression of α-tubulin. Non-transfected values are set to one.