Actin depolymerization in injured sciatic nerves prevents transfer of RNA into axons.

<p><b>A, C, E, G, I</b>, representative confocal images of BrU labeling at nodes of Ranvier. Bar = 10 µm. <b>B, D, F, H, J</b>, quantification of BrU fluorescence from 10 or more line scans across perinodal regions normalized to the mean of each linescan. Error bars represent standard errors. <b>A and B</b>, control BrU labeling without Latrunculin A; <b>C and D</b>, 0.07 µg/ml Latrunculin A during BrU labeling; <b>E and F</b>, 0.2 µg/ml; <b>G and H</b>, 0.6 µg/ml; <b>I and J</b>, 1.8 µg/ml. <b>K</b>, absolute BrU fluorescence intensities for the 8 bins at each edge combined (n = 304), representing RNA in the outer Schwann cell wrap, and the 20 bins in the center of each linescan (n = 380), representing RNA in the axon, for the control untreated and highest latrunculin A concentration (1.8 µg/ml) nerves. Error bars represent standard errors.