Characterization of myofiber derived from MyoD-hiPSCs.

<p>(<b>a</b>) Time course gene expression profile for undifferentiated and myogenic markers in B7 #9 MyoD-hiPSC clone with (gray bars) or without (black bars) Dox administration (n = 3). Data are listed as mean±S.D. The data were standardized by β-actin using teratoma. The data on d0 = 1 in undifferentiated markers, such as <i>OCT3/4</i>, <i>SOX2</i> and <i>NANOG</i>. The data on d7 = 1 in other analyses. *: <i>p</i><0.05, **: <i>p</i><0.01, respectively, between Dox(–) and Dox(+). (<b>b</b>) Intracellular localization of mitochondria in both undifferentiated and differentiated MyoD-hiPSCs. Scale bar = 20 µm. (<b>c</b>) Immunohistochemistry of differentiated MyoD-hiPSCs for mature myogenic markers, such as CK-M, creatine kinase muscle isoform, Skeletal muscle Actin, and DYSTROPHIN. Scale bar = 20 µm. (<b>d</b>) Heat map of global mRNA expression comparing undifferentiated hiPSC (sample 1) and differentiated myogenic cells (samples 2-5). (<b>e</b>) Myogenic gene profile and unsupervised clustering based on markers associated with myofibers for undifferentiated hiPSCs and differentiated myogenic cells. Red color indicates up-regulated genes and blue color indicates down-regulated genes in (<b>d</b>) and (<b>e</b>).