Luciferase activity of Bmp4Luc reporter constructs <i>in vitro</i>.

<p>(A) Mouse <i>Bmp4</i> is located on the long arm of mouse chromosome 14, and consists of four exons (IA, IB, II, III and IV) shown by boxes with two transcription start sites (TSS1, TSS2). Dark boxes show the coding sequence. The position of each element is counted from TSS1 as +1. Triangles denote splicing spans. (B) Luciferase activity of Bmp4Luc reporter constructs. NIT1 and NIH3T3 cells were transiently cotransfected with reporter plasmids and <i>Renilla</i> luciferase vector (pRL-TK) as a transfection control. The <i>x</i> axes show relative luciferase activities: firefly luciferase (FL) from the reporter plasmid was normalized to <i>Renilla</i> (RL) luciferase activity from the control vector. All data are the mean ± SE from three independent experiments. **Represents a significant difference (p<0.01) by Student’s <i>t</i>-test.