The proliferation of the splenocytes and number of IFN-γ⁺ T cells of the mice immunized by nano-Esat-6/3e-FL.

<p><b>A.</b> Splenocytes were cultured and stimulated with the Esat-6/3e (10 µg/ml) for 72 h. The stimulation index (SI) of splenocytes in the mice treated with different vaccines was calculated to determine the proliferation activity. <b>B.</b> The numbers of IFN-γ⁺ T cells from the splenocytes were quantified by ELISPOT assays. Data are one representative of three experiments and presented as mean value ± SD (n = 6). The statistics were performed with one-way ANOVA. <i>NS: P</i>>0.05; ***<i>P</i><0.001 versus the mice treated with nano-chitosan, nano-pIRES and nano-FL plasmids. The differences among other treatments were shown directly on the figure.