The CTL activity of the mice elicited by nano-Esat-6/3e-FL or other plasmids.

<p>Splenocytes from naive mice pulsed with (CFSEhigh) or without (CFSElow) peptides were transferred into the immunized mice. The representative histograms (<b>A</b>) and percentages (<b>B</b>) of specific lysis in the immunized mice were compared. Data are one representative results from three performed experiments, and presented as the mean ± SD (<i>n</i> = 6). <i>NS: P</i>>0.05; *<i>P</i><0.05; ***<i>P</i><0.001 versus the mice treated with nano-chitosan, nano-pIRES or nano-FL plasmids; the other differences among nano-Esat-6/3e-FL, nano-Esat-6/3e, Esat-6/3e-FL, nano-Esat-6, nano-Esat-6-FL and BCG treatments were showed on the figure directly.