Testing the central 392-bp <i>Hpa</i>I–<i>Nde</i>I part of scs (scs^m) inserted in either (A) direct or (B) reverse orientation for the ability to terminate transcription in the eyes.

<p>The UAS promoter is shown as the white rectangle marked “UAS.” “+GAL4” indicates that eye phenotypes in transgenic lines were examined after induction of GAL4 expression. In this case, N is the number of lines in which flies acquired a new w phenotype upon induction of GAL4. For other designations, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062690#pone-0062690-g001" target="_blank">Figures 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062690#pone-0062690-g002" target="_blank">2</a>.