GAS6 receptor expression by PCa cell lines.

<p>(A) FACS analysis for GAS6 receptors. The human PC3, VCaP, LNCaP, and DU145 PCa cell lines, human bone marrow endothelial cells (HBME), MG-63 human osteosarcoma cell line and primary human fetal osetoblasts (hFOB) were stained with antibodies targeting Axl, Tyro3 or Mer and the % expression were compared to IgG controls for each antibody for n = 3 samples. The data is expressed as avg. ± s.d. *<i>p</i><0.05 compared to expression of Axl for each cell type. (B). Western blot analysis for Tyro3. Since Tyro3 expression was not detected in (A) by FACS, validation by Western Blot of PCa cell lysates (30 µg) was performed. Lysates (1 µg) recovered from HEK293 cells which over expressed of human Tyro 3 (or vector control) were used as positive and negative controls for Tyro3 expression. Normalization of PCa cell extracts was evaluated by staining with antibody to ß-Actin (not shown).