Immunohistochemistry of the hypomorphic hearts.

<p>(A) Staining with an antibody specific for the phosphorylation of E-cadherin is strongly increased in the <i>Aof2</i>^2lox/2lox hearts compared to the control. The arrows indicate the regions (grey for heart wall, black for septum) from which the higher magnification images (on right) originate. (B) β-catenin localization is altered, with more of the protein present at the plasma membrane in <i>Aof2^2lox/2lox</i> hearts. (C) Lsd1 staining shows slightly decreased signals in <i>Aof2^2lox/2lox</i> hearts. (D) Staining with a non-specific IgG control antibody confirms the specificity of the staining. To minimize background, no counterstain was used. All photomicrographs constitute representative fields; magnification factor is provided above or beside the photographs.