Zymosan uptake by macrophages and DC.Assay of cytokine production.

<p>Macrophages were stimulated as indicated and after 24 hours the supernatants were collected for the assay of cytokines. (A) Inhibitory anti-CD32 and anti-dectin-1 Ab were used at a concentration of 10 µg/ml, 30 min prior to the addition of the stimuli. (B) The combination of a priming concentration of LPS and 10 mM ATP was used as a control of inflammasome activation. Results represent mean ± S.D. of 4 independent experiments in the case of TNFα (A) and IL-1β (B), and three experiments in the case of IL-23 (C) and IL-6 (D). *Indicates <i>p</i><0.05. In (C) and (D) comparison has been conducted between serum and M-CSF differentiated macrophages.