Activation of NF-κB proteins by LPS and zymosan.

<p>(A) Macrophages were treated for up to 5 h with 10 ng/ml LPS and RelA/p65 was assayed in the cytoplasm and nucleus. (B) Macrophages were primed for 3 h with LPS and then stimulated for different times. (B–C) The nuclear fractions were used for the assay of RelA/p65, c-Rel, and p50. (D) Binding of RelA/p65 and c-Rel, and histone H3 acetylation of the <i>ptgs2</i> promoter were assayed in the presence and absence of LPS priming 2 h after stimulation. These are representative experiments of at least two showing identical results.