Determination of erythrocyte peptide binding domains of PvTRAg33.5.

<p>(<b>A</b>) Schematic representation of eight non-overlapping peptides designed from M-PvTRAg33.5 and C-PvTRAg33.5 fragments. Amino acid sequence and name of each peptide along with residue numbers is shown. (<b>B</b>) Cell-ELISA showing erythrocyte binding affinity of these synthetic non overlapping peptides. Increasing concentrations of these peptides were allowed to bind with erythrocytes. Reaction with primary and secondary antibodies was carried out as described in Fig. 1B. Recombinant thioredoxin from <i>D. desulfuricans</i> was used as negative controls. Mean± SD value of absorbance from three experiments is plotted. SP, signal peptide; Int, intron.