Effect of CS exposure on NKT cell recruitment.

<p>A. Percentage of iNKT cells (TCRβ⁺ CD1d tetramer⁺) in CD45⁺ cells in the colonic mucosa of mice exposed or not to CS. Colonic tissues of several mice per group (3 to 5 animals) were pooled before cell extraction in order to obtain enough cell for cytometry analysis. On the left: a representative dot plot is shown. Numbers indicate iNKT cell percentage. On the right: Graph represents the mean value ± SEM of the iNKT cell percentage in the colonic mucosa according to CS exposure (n = 32 mice/condition from 3 independent experiments). Number on the graph represents p value. B. Vα14 mRNA expression in the colon as determined by real time qPCR analysis after normalization by the β-actin expression (17