CD8⁺ T cell Immunodominance is altered by the route of infection for VACV.

<p>(A–C) CD8⁺ T cell responses to VACV epitopes were determined in C57BL/6 mice infected with 1×10⁶ PFU of VACV WR by i.v., i.p., i.d., or s.c. injection seven days after infection. Data are from two or more experiments and total mice for each route were: i.v. 8, i.p. 16, i.d. 6 and s.c. 10. A) Means and SEM of percent of CD8⁺ T cells responding to each peptide. B) The fraction of all measured responses (sum of responses to all 15 VACV peptides) accounted for by B8₂₀-specific (IDE) and the sum of SDE-specific CD8⁺ T cells. C) Responses to VACV epitopes broken down into IDE and SDE as in panel B, but shown as total numbers of CD8⁺IFN-γ⁺ cells per spleen. (D–E) Responses to VACV and HSV gB₄₉₈ epitopes seven days after infection of mice with 1×10⁶ PFU of recombinant VACV-fullgB or VACV-ESminigB. Data are from two experiments with a total of 6 mice per virus. D) Means and SEM of percent of CD8⁺ T cells responding to each peptide after i.v. infection (10 of 14 VACV epitopes shown). E) Response to gB₄₉₈ shown as a fraction of the total measured epitope-specific responses (VACV+gB₄₉₈). Selected statistical significance denoted with p value over appropriate bars.