Wider spread but not longevity of antigen presentation is associated with systemic routes.

<p>A) Cytotoxicity in lymphoid organs of mice infected with 1×10⁶ PFU of VACV WR by the routes shown was determined by measuring the specific lysis of B8₂₀ epitope–pulsed splenocytes two days after infection. Data are from two or more experiments and total mice for each route were: i.d. 7, i.p. 9 and i.v. 7. Killing in the spleen was significantly lower for i.d. compared with i.p. (p<0.05) or i.v. (p<0.01) infected mice. B) B6.SJL mice were given 5×10⁶ OT-I CD8⁺ T cells (CD45.2⁺), then the following day infected with 1×10⁶ PFU of VACV NP-S-GFP by the routes shown. At 24 hours post-infection, expression of CD8, CD45.2 and CD69 were measured by flow cytometry on cells from spleens and LNs. Mean and SEM of percent of OT-I CD8⁺ cells expressing CD69 is shown. Data are from two or more experiments and total mice for each route were: i.d. 5, i.p. 5 and i.v. 3. Priming of OT-I in the spleen was significantly (p<0.05) lower for i.d. compared with i.p. (p<0.01) or i.v. (p<0.001) infected mice. C) B6.SJL mice were infected with 1×10⁶ PFU of VACV NP-S-GFP by the routes shown then given 5 × 10⁶ OT-I CD8⁺ T cells (CD45.2⁺) 1, 3 and 5 days later. Expression of CD8, CD45.2 and CD69 were measured by flow cytometry on cells from spleens and LNs 24 hours after transfer to give times 2, 4 and 6 as shown on the graph. Means and SEM of percent of OT-I CD8⁺ cells expressing CD69 are shown.